Commercial house: Applied Biosystems
Unit: 1
Location: Room 312
The Applied Biosystems 7900HT Fast Real-Time PCR System is a second-generation sequence de tection system instrument designed for automated, high-throughput detection of fluorescent P CR-related chemistries. The instrument is capable of real-time, end-point, and dissociation curve analysis of assays arrayed on multiple formats. The 7900HT instru ment is optimized for use with Applied Biosystems chemistr ies for nucleic acid quan tification and detection.
This instrument is accessible to users but shared with the staff of the service, so its use is adjusted to comply with a series of rules (see below).
- Best practices and reservation of the ABI PRISM 7900HT equipment:
1- Time zone 8-15 h: reservations can be made (preferably a maximum of 3 hours) from 3:00 p.m. two days before the reservation date (examples: for the morning of a Wednesday, it can be reserved from the 3:00 pm on Monday, for the morning of a Monday, it can be booked from 3:00 pm on the previous Thursday).
2- Time zone of 15 h and up: - Reservations can be made with one week in advance (maximum).
- The reservation time will be limited to 3 h per research group and day, from Monday to Friday from 8 a.m. to 6 p.m. (peak hours). Outside of that period, additional reservations can be made.
- Exception to the aforementioned: from 12 noon on the day before the date of the reservation, additional reservations may be made without a time limit.
3- It is allowed to put runs without the obligation to stay in the center to turn off the equipment. However, the heat generated by the equipment during the night makes the work in the common area of the laboratory the next day much more uncomfortable for all (apart from the economic and ecological cost in air conditioning), so we request a use as rational as possible of this measure.
4- Remember the possibility of using chemical “fast” that significantly reduce the duration of the runs.
5- Adjust the duration of the reserve strictly to that of the run, avoiding margins of “security” or programming / analysis (as a practical example, half an hour of margin in each user reduces daily availability in one or two runs). The programming and analysis softwares are at your disposal in our service so that you can install them on your computers.
6- Respect the reserves with the maximum timeliness, since the use of shared equipment outside of the reserved schedule affects the programming of other colleagues’ work.
7- Warn the next user if, in spite of what has been commented previously, you delay or advance with respect to your theoretical reserve schedule.
8- Avoid preventive reserves because they can generate cancellations that often can not be used.
9- Notwithstanding the foregoing, notify cancellations as soon as possible to maximize the probability that the hole can be used by another user.
Thanks for your cooperation.
7900HT Fast Real-Time PCR System with 384-Well Block Module and Automation Accessory
The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability. The optional, integrated Automation Accesso