qPCR, RT-qPCR

Commercial house: Biosan

Unit: 1

Location: Room 312

Commercial house: Eppendorf

Unit: 1

Location: Room 312

The epMotion 5075 LH is the perfect solution for more complex pipetting tasks such as qPCR experiments in 384-well plates. The 12 lid positions and the option for automatically changing pipetting tools make the epMotion 5075 LH the most flexible automated pipetting system.

The instrument is used by our staff to carry out RT, conventional PCR and qPCR reactions requested by internal and external users.

Commercial house: Applied Biosystems

Unit: 1

Location: Room 312

Best practices and booking of the ABI PRISM 7900HT equipment:

1. The maximum advance reservation time of this equipment is one week (both in the morning or in the afternoon).
2. Bookings are limited up to 3 hours per research group per day, from Monday to Friday from 8 a.m. to 6 p.m. (peak hours). Additional bookings, outside of that time-frame, can be made. Exception to the aforementioned: from 12 noon on the day before the date of the reservation, additional reservations may be made without a time limit.
3. It is allowed to start the runs without the obligation to remain in the center to turn off the equipment after use it. However, it is important to consider the economic and ecological cost involved. Please also note that the heat emitted when the device is switched on during the night, makes uncomfortable to work the following day. We would therefore ask for your cooperation to a more rational use of the procedure.
4. Remember to use chemical “fast” where possible as it significantly reduces the duration of the runs.
5. Adjust the duration of the reservation strictly to that of the run, avoiding margins of «security» or programming / analysis (as a practical example, half an hour of margin in each user reduces daily availability in one or two runs). The programming and analysis software we put at your disposal for installation on your computer.
6. Bookings must be respected at the time the user made its reservation, since the shared use of the equipment, outside the booked hours, affects the work schedule of your colleagues.
7. Let the next user know, if in spite of all the measures explained beforehand, you are going to arrive early or late to your scheduled reservation time.
8. Avoid extra bookings (those to prevent) because those can generate cancellations and no one can take advantage of.
9. In case you cancel the booking, please notify as soon as possible to allow rescheduling by other user.

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Commercial house: Roche

Unit: 3

Location: Room 264

The Service has three of these devices, located in room 264, on the second floor. These devices are designed to carry out PCR and RT-PCR tests in real time by using different types of fluorescent labels. A blue light emits diode at 470 nm and three signal detector channels at 530 nm (green), 640 nm (red) and 710 nm (red). The reactions are carried out in glass capillaries (max 32 per race). The procedure takes place on the computer screen in real time.

More information about LightCycler

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Commercial house: BioRad

Unit: 2 (one is available to the users through the on-line booking plataform)

Location: Room 312

The CFX384 Touch Real-Time PCR Detection System is a powerful, precise real-time PCR instrument in a 384-well format designed for researchers who require both ease of use and rapid, high-throughput data acquisition. The CFX384 Touch System uses accurate temperature control and sensitive, 4-color detection optics to deliver reliable and repeatable qPCR results for singleplex or multiplex reactions.

Set up runs quickly and monitor amplification traces in real time on the integrated LCD touch screen or use CFX Maestro™ Software to easily and intuitively design your experiments and analyze results from a connected computer. With up to four-target detection, unsurpassed thermal cycler performance, unrivaled stand-alone functionality, and powerful software, the CFX384 Touch System was designed to advance your qPCR.

The instruments are used by the service staff to perform experiments by internal and external users.

Best practices and booking of the CFX384 Touch Real-Time PCR equipment:

1. The maximum advance reservation time of this equipment is one week (both in the morning or in the afternoon).
2. Bookings are limited up to 3 hours per research group per day, from Monday to Friday from 8 a.m. to 6 p.m. (peak hours). Additional bookings, outside of that time-frame, can be made. Exception to the aforementioned: from 12 noon on the day before the date of the reservation, additional reservations may be made without a time limit.
3. It is allowed to start the runs without the obligation to remain in the center to turn off the equipment after use it. However, it is important to consider the economic and ecological cost involved. Please also note that the heat emitted when the device is switched on during the night, makes uncomfortable to work the following day. We would therefore ask for your cooperation to a more rational use of the procedure.
4. Remember to use chemical “fast” where possible as it significantly reduces the duration of the runs.
5. Adjust the duration of the reservation strictly to that of the run, avoiding margins of «security» or programming / analysis (as a practical example, half an hour of margin in each user reduces daily availability in one or two runs). The programming and analysis software we put at your disposal for installation on your computer.
6. Bookings must be respected at the time the user made its reservation, since the shared use of the equipment, outside the booked hours, affects the work schedule of your colleagues.
7. Let the next user know, if in spite of all the measures explained beforehand, you are going to arrive early or late to your scheduled reservation time.
8. Avoid extra bookings (those to prevent) because those can generate cancellations and no one can take advantage of.
9. In case you cancel the booking, please notify as soon as possible to allow rescheduling by other user.

More information about CFX384 Touch Real-Time PCR

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